منابع مشابه
Carbonic anhydrase under pressure
Investigations of the rapid enzyme carbonic anhydrase have now been extended by crystallographic analysis at high CO2 pressures to examine the movements of water molecules in different steps of the catalysis. The rate of catalysis seems well explained by the assembled observations.
متن کاملGastric Carbonic Anhydrase In
Davenport and Fisher (1938) reported the discovery DOGS P School of Medicine of large amounts of carbonic anhydrase in the gastric mucosa of cats, rats and rabbits. Davenport (1939) demonstrated that carbonic anhydrase is present in high concentration in the parietal cells and in lower concentration in the cells of the surface epithelium of the gastric mucosa of cats and rats. When those invest...
متن کاملCarbonic anhydrase in human platelets.
The carbonic anhydrase activity of human platelets was investigated by measuring the kinetics of CO2 hydration in supernatants of platelet lysates by using a pH stopped-flow apparatus. An average carbonic anhydrase concentration of 2.1 microM was determined for pellets of human platelets. Analysis of the kinetic properties of this carbonic anhydrase yielded a Km value of 1.0 mM, a catalytic-cen...
متن کاملCarbonic anhydrase in marine algae.
An electrometric system for determination of carbonic anhydrase activity was constructed. Enzyme activity was assayed in homogenates of marine macroscopic Chlorophyta, Rhodophyta, and Phaeophyta. Plants surveyed included Ulva expansa (Setchell) Setchell and Gardner, Codium fragile (Suringar) Hariot, Enteromorpha sp., Chaetomorpha torta (Farlow) McClatchie (Chlorophyta); Laurencia papillosa (Gre...
متن کاملThermodynamics of Carbonic Anhydrase Catalysis
The COZ hydration and HC03dehydration activities of human red cell carbonic anhydrase isozymes B and C (HCAB and HCAC) have been studied as a function of temperature from 0” to 37°C. The Arrhenius plots of In kcat versus 1/T are linear for both isozymes in both hydration and dehydration reactions, indicating that the rate-determining steps remain unchanged over this temperature range. The 37°C ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1942
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)72518-0